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- Title
Novel Extracellular Electron Transfer Channels in a Gram-Positive Thermophilic Bacterium.
- Authors
Gavrilov, Sergey N.; Zavarzina, Daria G.; Elizarov, Ivan M.; Tikhonova, Tamara V.; Dergousova, Natalia I.; Popov, Vladimir O.; Lloyd, Jonathan R.; Knight, David; El-Naggar, Mohamed Y.; Pirbadian, Sahand; Leung, Kar Man; Robb, Frank T.; Zakhartsev, Maksim V.; Bretschger, Orianna; Bonch-Osmolovskaya, Elizaveta A.
- Abstract
Biogenic transformation of Fe minerals, associated with extracellular electron transfer (EET), allows microorganisms to exploit high-potential refractory electron acceptors for energy generation. EET-capable thermophiles are dominated by hyperthermophilic archaea and Gram-positive bacteria. Information on their EET pathways is sparse. Here, we describe EET channels in the thermophilic Gram-positive bacterium Carboxydothermus ferrireducens that drive exoelectrogenesis and rapid conversion of amorphous mineral ferrihydrite to large magnetite crystals. Microscopic studies indicated biocontrolled formation of unusual formicary-like ultrastructure of the magnetite crystals and revealed active colonization of anodes in bioelectrochemical systems (BESs) by C. ferrireducens. The internal structure of micron-scale biogenic magnetite crystals is reported for the first time. Genome analysis and expression profiling revealed three constitutive c -type multiheme cytochromes involved in electron exchange with ferrihydrite or an anode, sharing insignificant homology with previously described EET-related cytochromes thus representing novel determinants of EET. Our studies identify these cytochromes as extracellular and reveal potentially novel mechanisms of cell-to-mineral interactions in thermal environments.
- Subjects
THERMOPHILIC bacteria; GRAM-positive bacteria; CHARGE exchange; MAGNETITE crystals; MAGNETITE; CYTOCHROMES; CYTOCHROME c; HOMOLOGY (Biochemistry)
- Publication
Frontiers in Microbiology, 2021, Vol 11, pN.PAG
- ISSN
1664-302X
- Publication type
Article
- DOI
10.3389/fmicb.2020.597818