We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Contralateral spreading of substances following intratympanic nanoparticle-conjugated gentamicin injection in a rat model.
- Authors
Lee, Sang-Yeon; Kim, Jeonghyo; Oh, Sangjin; Jung, Gaon; Jeong, Ki-Jae; Tan Tran, Van; Hwang, Dajeong; Kim, SungIl; Song, Jae-Jin; Suh, Myung-Whan; Lee, Jaebeom; Koo, Ja-Won
- Abstract
This study was performed to investigate the Eustachian tube as a potential route for contralateral spreading following intratympanic nanoparticle (NP)-conjugated gentamicin injection in a rat model. Sprague–Dawley rats were divided into three groups and substances were injected in the right ear: group 1 (fluorescent magnetic nanoparticles [F-MNPs], n = 4), group 2 (F-MNP-conjugated gentamicin [F-MNP@GM], n = 2), and control group (no injections, n = 2). T2-weighted sequences corresponding to the regions of interest at 1, 2, and 3 h after intratympanic injection were evaluated, along with immunostaining fluorescence of both side cochlea. The heterogeneous signal intensity of F-MNPs and F-MNP@GM on T2-weighted images, observed in the ipsilateral tympanum, was also detected in the contralateral tympanum in 4 out of 6 rats, recapitulating fluorescent nanoparticles in the contralateral cochlear hair cells. Computational simulations demonstrate the contralateral spreading of particles by gravity force following intratympanic injection in a rat model. The diffusion rate of the contralateral spreading relies on the sizes and surface charges of particles. Collectively, the Eustachian tube could be a route for contralateral spreading following intratympanic injection. Caution should be taken when using the contralateral ear as a control study investigating inner-ear drug delivery through the transtympanic approach.
- Subjects
GENTAMICIN; ANIMAL models in research; MAGNETIC nanoparticles; HAIR cells; EUSTACHIAN tube
- Publication
Scientific Reports, 2020, Vol 10, Issue 1, pN.PAG
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/s41598-020-75725-y