We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Phage Mu transposition immunity reflects supercoil domain structure of the chromosome.
- Authors
Manna, Dipankar; Higgins, N. Patrick
- Abstract
Transposition immunity is the negative influence that the presence of one transposon sequence has on the probability of a second identical element inserting in the same site or in sites nearby. A transposition-defective Mu derivative (Mud Jr1) produced transposition immunity in both directions from one insertion point in the Salmonella typhimurium chromosome. To control for the sequence preference of Mu transposition proteins, Tn10 elements were introduced as targets at various distances from an immunity-conferring Mud Jr1 element. Mu transposition into a Tn10 target was not detectable when the distance of separation from Mud Jr1 was 5 kb, and transposition was unencumbered when the separation was 25 kb. Between 5 kb and 25 kb, immunity decayed gradually with distance. Immunity decayed more sharply in a gyrase mutant than in a wild-type strain. We propose that Mu transposition immunity senses the domain structure of bacterial chromosomes.
- Subjects
BACTERIOPHAGE mu; CHROMOSOMES; CHROMOSOMAL translocation
- Publication
Molecular Microbiology, 1999, Vol 32, Issue 3, p595
- ISSN
0950-382X
- Publication type
Article
- DOI
10.1046/j.1365-2958.1999.01377.x