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- Title
Association of cannabinoid type 1 receptor and fatty acid amide hydrolase genetic polymorphisms in Chinese patients with irritable bowel syndrome.
- Authors
Jiang, Yingjie; Nie, Yuqiang; Li, Yuyuan; Zhang, Long
- Abstract
Background and Aim The endocannabinoid system is involved in the pathophysiology of irritable bowel syndrome ( IBS). Here, we investigated whether genetic variants of the cannabinoid type 1 receptor ( CNR1) and fatty acid amide hydrolase ( FAAH) are associated with the pathogenesis of IBS. Methods In total, 292 patients with IBS and 298 healthy controls were enrolled. Polymerase chain reaction ( PCR) and DNA sequencing were applied to determine the genotyping of polymorphic triplet AAT repeats located at the 3′-end of the CNR1 gene. The single nucleotide polymorphism ( SNP) C385A at the FAAH gene (rs324420) was determined by PCR using Taq Man SNP Genotyping Assay Sets. Results A total of eight alleles with AAT triplet repeats in the CNR1 gene were detected. The alleles were divided into two groups (≤ 10 and > 10) and three genotypes (≤ 10/≤ 10, ≤ 10/> 10, and > 10/> 10). The frequency of > 10 alleles was significantly higher in the IBS group (90.6%) when compared with the control group {81.7%, P < 0.001, odds ratio ( OR) (95% confidence interval [ CI]) = −0.128}. In addition, the genotypes > 10/> 10 were significantly associated with IBS ( P < 0.001, OR [95% CI] = −0.163). The frequency of the A allele and the distribution of the AA genotype in the FAAH gene in the IBS group were not significantly different from those in the control group ( P > 0.05), even though the frequency of the AA genotype was lower in the IBS group (1.0%) than that in the control group (3.4%, P = 0.089, OR [95% CI] = 3.345). Conclusions The variation in the ( AAT)n repeat of the CNR1 gene conferred an increased risk for developing IBS, while rs324420 ( C385) in the FAAH gene was not associated with IBS pathogenesis.
- Subjects
CANNABINOID receptors; FATTY acids; SINGLE nucleotide polymorphisms; GENETIC polymorphisms; IRRITABLE colon; PATHOLOGICAL physiology
- Publication
Journal of Gastroenterology & Hepatology, 2014, Vol 29, Issue 6, p1186
- ISSN
0815-9319
- Publication type
Article
- DOI
10.1111/jgh.12513