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- Title
Cytoprotective effects of fruit pulp of Eugenia jambolana on H<sub>2</sub> O<sub>2</sub>-induced oxidative stress and apoptosis in rat Leydig cells in vitro.
- Authors
Anand, H.; Misro, M. M.; Sharma, S. B.; Prakash, S.
- Abstract
This study was undertaken to investigate the cytoprotective effect of the fruit pulp of Eugenia jambolana (50-250 μg ml−1) against the damage induced by H2 O2 (100 μ m) exposure to Leydig cells in vitro. Cell survival with extract was found comparable to similar effects by N-acetyl- l-cysteine. H2 O2-induced rise in thiobarbituric acid reactive substance formation and decline in the activity and expression of antioxidant enzymes like superoxide dismutase, catalase and glutathione-s-transferase were effectively checked. Cellular glutathione and total antioxidant capacity demonstrated significant improvement. The increase in expression of inducible nitric oxide ( NO) synthase leading to NO production was successfully countered. Co-treatment of the extract helped in the down-regulation of caspase-3 and poly- ADP-ribose polymerase resulting in a significant reduction in Leydig cell apoptosis induced by H2 O2. Upstream marker proteins of extrinsic (caspase-8, Fas, Fas L) and intrinsic (caspase-9) pathway of metazoan apoptosis were identically down-regulated. The Bcl-2 family of proteins, though, remained unaffected. The extract also positively modulated the other marker proteins like c- Jun NH2-terminal kinase, p38, Akt, nuclear factor-κ B, c- Fos, cellular FLICE-inhibitory protein, cyclooxygenase-2 and p53. Taken together, the above-mentioned findings establish the anti-oxidative and anti-apoptotic potency of the extract that ameliorates the H2 O2-induced adverse effects on rat Leydig cells in vitro.
- Subjects
CYTOPROTECTION; EUGENIA; OXIDATIVE stress; APOPTOSIS; LEYDIG cells; GLUTATHIONE; ANTIOXIDANTS
- Publication
Andrologia, 2013, Vol 45, Issue 3, p145
- ISSN
0303-4569
- Publication type
Article
- DOI
10.1111/j.1439-0272.2012.01323.x