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- Title
Utilization of Cottonseed Meal Protein Hydrolysate by Crustaceans: Insights on Growth Performance, Protein Turnover, and Metabolism in Chinese Mitten Crab Eriocheir sinensis.
- Authors
He, Chaofan; Liu, Wenbin; Zhang, Ling; Chen, Weiliang; Liu, Zishang; Qian, Xiangyu; Li, Xiangfei
- Abstract
Simple Summary: Advancing the utilization of plant proteins is of great significance for aquaculture. Here, the effects of cottonseed meal protein hydrolysate (CPH) on the growth performance and protein metabolism of Chinese mitten crab were investigated. CPH enhanced protein deposition through the activation of the IGF-1/Akt/TOR and IGF-1/Akt/FoxO1 pathways, thereby promoting growth performance. This facilitates the development of effective nutritional interventions to promote the growth of crustaceans. Plant protein hydrolysates could enhance the growth performance and diet utilization of aquaculture species. The mechanisms underlying their beneficial effects, however, remain unclear. The purpose of this study was to appraise the effects of cottonseed meal protein hydrolysate (CPH) supplementation on the growth performance, amino acid profiles, and protein turnover and metabolism of Eriocheir sinensis. A total of 240 crabs (initial weight: 37.32 ± 0.38 g) were randomly assigned to six groups, and fed six iso-protein feeds supplemented with varying levels of 0% (the control group), 0.2%, 0.4%, 0.8%, 1.6% and 3.2% of CPH. These diets were continuously fed to the crabs for 12 weeks. The findings demonstrated that, compared with the control group, adding 0.4–0.8% CPH to the diet significantly increased the specific growth rate, nitrogen retention efficiency, hepatopancreas index, body crude protein content, hepatopancreas alanine aminotransferase and glutamine synthetase activities, hemolymph total protein content, the hepatopancreas transcription of S6 kinase-poly-peptide 1, and the hepatopancreas protein levels of insulin-like growth factor-1 (IGF-1), protein kinase B (Akt), and target of rapamycin (TOR) of crabs. In contrast, when the dose of dietary CPH reached 3.2%, the forkhead box O1 (FoxO1) protein expression showed a significant decrease compared with the control group. In addition, CPH supplementation also increased the amount of amino acids and free amino acids in hepatopancreas and hemolymph, respectively. Together, these findings demonstrated that dietary supplementation of 0.4–0.8% CPH could activate the IGF-1/Akt/TOR pathway of E. sinensis, thereby improving growth performance, protein synthesis, and utilization. For cost considerations, the recommended dietary dose of CPH for E. sinensis is 0.8%. Due to the above benefits, CPH has the potential to be used as a growth promoter for other aquatic animals, especially crustaceans.
- Subjects
CHINESE mitten crab; CRUSTACEAN growth; GLUTAMINE synthetase; COTTONSEED meal; PROTEIN hydrolysates; FISH feeds; COTTONSEED; PLANT proteins
- Publication
Animals (2076-2615), 2023, Vol 13, Issue 23, p3631
- ISSN
2076-2615
- Publication type
Article
- DOI
10.3390/ani13233631