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- Title
Femtosecond Laser Axotomy in <i>Caenorhabditis elegans</i> and Collateral Damage Assessment Using a Combination of Linear and Nonlinear Imaging Techniques.
- Authors
Santos, Susana I. C. O.; Mathew, Manoj; Olarte, Omar E.; Psilodimitrakopoulos, Sotiris; Loza-Alvarez, Pablo
- Abstract
In this work highly localized femtosecond laser ablation is used to dissect single axons within a living Caenorhabditis elegans (C. elegans). We present a multimodal imaging methodology for the assessment of the collateral damage induced by the laser. This relies on the observation of the tissues surrounding the targeted region using a combination of different high resolution microscopy modalities. We present the use of Second Harmonic Generation (SHG) and Polarization Sensitive SHG (PSHG) to determine damage in the neighbor muscle cells. All the above is done using a single instrument: multimodal microscopy setup that allows simultaneous imaging in the linear and non-linear regimes and femtosecond-laser ablation.
- Subjects
FEMTOSECOND lasers; CAENORHABDITIS elegans; NONLINEAR imaging sensors; LINEAR systems; LASER ablation; AXONS; HIGH resolution imaging
- Publication
PLoS ONE, 2013, Vol 8, Issue 3, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0058600