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- Title
The Neuroprotective Effect of Cannabinoid Receptor Agonist (WIN55,212-2) in Paraoxon Induced Neurotoxicity in PC12 Cells and N-methyl-D-aspartate Receptor Interaction.
- Authors
Hashemi, Mansoureh; Bahrami, Farideh; Sahraei, Hedayat; Golmanesh, Leila; Sadri, Soheil
- Abstract
Objective: Considering that cannabinoids protect neurons against neurodegeneration, in this study, the neuroprotective effect of WIN55,212-2 in paraoxon induced neurotoxicity in PC12 cells and the rote of the N-methyl-D-aspartate (NMDA) receptor were evaluated. Materials and Methods: In this study PCI2 cells were maintained in Dulbeccos modified eagles medium (DMEM+F12) culture medium supplemented with 10% fetal bovine serum. The cells were treated with paraoxon (200 μM) in the presence or absence of WIN55,212-2 (0.1 μM), NMDA receptor agonist NMDA (100 μM), cannabinoid receptor antagonist AM251 and NMDA receptor antagonist MK801 (1 μM)at 15 minutes intervals. After 48 hours of exposure, cellular viability and protein expression of the CBI receptor were evaluated in PC12 cells. Results: Following the exposure of PC12 cells to paraoxon (200 μM), a reduction in cell survival and protein level of the CBI receptorwas observed (p<0.01 ). Treatment-of the cells with WIN55,212-2 (0.1 μM) and NMDA (100 μM) priorto paraoxon exposure significantly elevated cell survival and protein level of the CB1 receptor (p<0.01). Also, AM251 (1 μM) did not inhibit the cell survival and protein level of the CB1 receptor increase induced by WIN55,212-2 (p<0.001). However, MK801 (1 μM) did inhibit cell survival and protein expression of the CB1 receptor increase induced by NMDA (p<0.001). Conclusion: The results indicate that WIN55,212-2 and NMDA protect PC12 cells against paraoxon induced toxicity. In addition, the neuroprotective effect of WIN55,212-2 and NMDA was cannabinoid receptor-independent and NMDA receptor dependent, respectively.
- Subjects
CYTOLOGICAL research; NEUROPROTECTIVE agents; CANNABINOIDS; PARAOXONASE; NEUROTOXICOLOGY; CELL culture
- Publication
Yakhteh Medical Journal, 2010, Vol 12, Issue 2, p183
- ISSN
1561-4921
- Publication type
Article