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- Title
Structure and function of urea amidolyase.
- Authors
Jing Zhao; Li Zhu; Chen Fan; Yi Wu; Song Xiang
- Abstract
Urea is the degradation product of a wide range of nitrogen containing bio-molecules. Urea amidolyase (UA) catalyzes the conversion of urea to ammonium, the essential first step in utilizing urea as a nitrogen source. It is widely distributed in fungi, bacteria and other microorganisms, and plays an important role in nitrogen recycling in the biosphere. UA is composed of urea carboxylase (UC) and allophanate hydrolase (AH) domains, which catalyze sequential reactions. In some organisms UC and AH are encoded by separated genes. We present here structure of the Kluyveromyces lactis UA (KlUA). The structure revealed that KlUA forms a compact homo-dimer with a molecular weight of 400 kDa. Structure inspired biochemical experiments revealed the mechanism of its reaction intermediate translocation, and that the KlUA holo-enzyme formation is essential for its optimal activity. Interestingly, previous studies and ours suggest that UC and AH encoded by separated genes probably do not form a KlUA-like complex, consequently they might not catalyze the urea to ammonium conversion as efficiently.
- Subjects
UREA; AMMONIUM; CARBOXYLASES; BIOTIN carboxylase; BIOMOLECULES
- Publication
Bioscience Reports, 2018, Vol 38, Issue 1, p1
- ISSN
0144-8463
- Publication type
Article
- DOI
10.1042/BSR20171617