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- Title
Transcriptomic analysis of Listeria monocytogenes biofilm formation at different times.
- Authors
Huitian Gou; Qihang Cao; Zijian Wang; Yuanyuan Liu; Yanan Sun; Huiling Wei; Chen Song; Changqing Tian; Yanquan Wei; Huiwen Xue
- Abstract
Biofilm (BF) formation is a considerable obstacle to the effective control of Listeria monocytogenes (LM). In this study, we used transcriptomics to analyze LM BF and planktonic bacteria at different stages of BF formation and growth to compare differential gene expression between the 2. We identified 1588, 1517, and 1462 differentially expressed genes (DEGs) when early formation BF and planktonic bacteria were compared at 12, 24, and 48 h, respectively. Among these, 1123 DEGs were shared across the 3 data pool. Gene Ontology functional enrichment and Kyoto Encyclopedia of Genes and Genomes pathway analyses demonstrated significant changes associated with the phosphotransferase system, the microbial metabolism in diverse environments, the flagella assembly, the bacterial chemotaxis, the bacterial secretion, the quorum sensing, and the 2-component system. The top 5 upregulated DEGs were lmo0024, lmo0374, lmo0544, hly, and lmo2434. The top 5 downregulated DEGs were lmo2192, lmo1211, cheY, lmo0689, and secY. After real-time quantitative polymerase chain reaction, the expression of these 10 DEGs were consistent with the results of the transcriptomic sequence. This research lays the foundation for further studies on mechanisms regulating BF formation and will help to identify BF inhibitors to reduce the risk of LM infection.
- Subjects
LISTERIA monocytogenes; BIOFILMS; GENE expression in bacteria; PHOSPHOTRANSFERASE genetics; BACTERIAL flagella; QUORUM sensing; POLYMERASE chain reaction
- Publication
Canadian Journal of Veterinary Research / Revue Canadienne de Recherche Vétérinaire, 2023, Vol 87, Issue 1, p59
- ISSN
0830-9000
- Publication type
Article