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- Title
Inhibition of trypsin-induced mast cell activation by water fraction of Lonicera japonica.
- Authors
Kang, Ok-Hwa; Choi, Yeon-A; Park, Hye-Jung; Lee, Joo-Young; Kim, Dae-Ki; Choi, Suck-Chei; Kim, Tae-Hyun; Nah, Yong-Ho; Yun, Ki-Jung; Choi, Suck-Jun; Kim, Young-Ho; Bae, Ki-Hwan; Lee, Young-Mi
- Abstract
Lonicera japonica Thunb.(Caprifoliaceae) has long been known as an anti-inflammatory. In the present study, the effect of water fraction of Lonicera japonica (LJ) on trypsin-induced mast cell activation was examined. HMC-1 cells were stimulated with trypsin (100 nM) in the presence or absence of LJ (10, 100, and 1000 μg/mL). TNF-α and tryptase production were measured by enzyme-linked immunosorbent assay (ELISA) and reverse transcription-PCR. Extracellular signal-regulated kinase (ERK) phosphorylation was assessed by Western blot. Trypsin activity was measured by using Bz-DL-Arg-p-nitroanilide (BAPNA) as substrate. LJ (10, 100, and 1000 μg/mL) inhibited TNF-α secretion in a dose-dependent manner. LJ (10, 100, and 1000 μg/mL) also inhibited TNF-α and tryptase mRNA expression in trypsin-stimulated HMC-1. Furthermore, LJ inhibited trypsin-induced ERK phosphorylation. However, LJ did not affect the trypsin activity even 1000 μg/mL. These results indicate that LJ may inhibit trypsin-induced mast cell activation through the inhibition of ERK phosphorylation than the inhibition of trypsin activity.
- Publication
Archives of Pharmacal Research, 2004, Vol 27, Issue 11, p1141
- ISSN
0253-6269
- Publication type
Article
- DOI
10.1007/BF02975120