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- Title
Creation of Functionally Diverse Chimerical α-Glucosidase Enzymes by Swapping Homologous Gene Fragments Retrieved from Soil DNA.
- Authors
Wang, Qiuyan; Yin, Xiaopu; Ma, Lifang; Pei, Xiaolin; Du, Pengfei; Li, Chenglu; Xie, Tian; Yu, Lei; Yu, Li
- Abstract
α-Glucosidase (XcG) from Xanthomonas campestris is an interesting enzyme due to its ability to catalyze transglycosylation reactions using maltose, rather than expensive nucleotide-activated sugars. In this study, two chimerical enzymes, XcG-A and XcG-B, were created by substituting the corresponding region of the XcG gene with gene fragments retrieved from metagenomic DNA samples. The enzymatic characterization results revealed that XcG-A exhibited a significant greater transglycosylation capacity for α-arbutin production and a significantly improved ability to produce α-arbutin-α-glycosides than XcG and XcG-B.
- Subjects
METAGENOMICS; ALPHA-glucosidases; ENZYMES; ARBUTIN; XANTHOMONAS campestris; POLYMERASE chain reaction
- Publication
Indian Journal of Microbiology, 2015, Vol 55, Issue 1, p114
- ISSN
0046-8991
- Publication type
Article
- DOI
10.1007/s12088-014-0493-5