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- Title
Bovine serum albumin adsorption on passivated porous silicon layers.
- Authors
Tay, L.; Rowell, N. L.; Poitras, D.; Fraser, J. W.; Lockwood, D. J.; Boukherroub, R.
- Abstract
Hydrogen-terminated porous silicon (pSi-H) films were fabricated through electrochemical anodization of crystalline silicon in hydrofluoric-acid-based solutions. The pSi-H surface was chemically functionalized by thermal reaction with undecylenic acid to produce an organic monolayer covalently attached to the silicon surface through Si—C bonds and bearing an acid terminal group. Bovine serum albumin (BSA) was adsorbed onto such surface-modified pSi structures. The resulting surfaces were characterized using scanning electron microscopy (SEM), reflection FT-IR spectroscopy, and ellipsometry. SEM showed that the porous films were damaged and partially lifted off the silicon substrate after a prolonged BSA adsorption. Ellipsometry analysis revealed that the BSA penetrated ∼1.3 µm into the porous structure. The film damage is likely a result of BSA anchoring itself tightly through strong electrostatic interaction with the acid-covered Si sidewalls. A change in surface tension during BSA film formation then causes the pSi layer to buckle and lift off the underlying Si substrate. FT-IR results from the undecylenic-acid-modified pSi surfaces before and after BSA adsorption showed the presence of strong characteristic amide I, II, and III vibrational bands after BSA adsorption. The surface properties of the pSi matrix and its interactions with BSA are examined in this study.
- Subjects
POROUS silicon; ELLIPSOMETRY; PROTEINS; SERUM albumin; SCANNING electron microscopy
- Publication
Canadian Journal of Chemistry, 2004, Vol 82, Issue 10, p1545
- ISSN
0008-4042
- Publication type
Article
- DOI
10.1139/V04-129