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- Title
Apical Periodontitis Healing Following Treatment is Impacted by Root Canal Sealer Composition: An in Vivo and in Vitro Investigation.
- Authors
Bezerra da Silva, Léa Assed; Cortês Bonifácio, Kléber; Souza Liévana, Fernanda; Gonçalves Martins, Giovana; Silva-Herzog Flores, Daniel; Roberto Leonardo, Mário; Bezerra da Silva, Raquel Assed; Garcia Paula-Silva, Francisco Wanderley
- Abstract
Objective: To evaluate the periapical healing following root canal treatment in teeth with apical periodontitis (in vivo) and the cytotoxic potential of root canal sealers in vitro. Material and Methods: Apical periodontitis was induced in 60 dogs' teeth and root canals were filled with Sealapex (40 roots), EndoREZ (40 roots), intracanal dressing (20 roots), or left untreated (20 roots). After 30 and 90 days, histopathological analyses were made. In vitro, J774.1 macrophages were stimulated with root canal sealers extracts, cytotoxicity was assessed using lactate dehydrogenase assay, and qRT-PCR was used to analyze TNF-α gene expression. Results: In vivo, smaller apical periodontitis and lower inflammatory cell infiltrate were found in teeth treated with Sealapex compared to EndoREZ. In vitro, EndoREZ was cytotoxic and induced TNF-α gene expression by macrophages differently from Sealapex. Conclusion: Sealapex allowed improved tissue repair following root canal treatment in teeth with apical periodontitis compared to EndoREZ. Synthesis of TNF-α induced by LPS was enhanced by EndoREZ, whereas Sealapex prevented pro-inflammatory gene expression.
- Subjects
PIT &; fissure sealants (Dentistry); PERIAPICAL periodontitis; ROOT canal treatment; HEALING; DENTAL pulp cavities
- Publication
Pesquisa Brasileira em Odontopediatria e Clinica Integrada, 2022, Vol 22, p1
- ISSN
1519-0501
- Publication type
Article
- DOI
10.1590/pboci.2022.058