We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Detection of protein coatings on nanoparticles surfaces by ToF-SIMS and advanced electron microscopy.
- Authors
Neunzehn, Jörg; Draude, Felix; Golla‐Schindler, Ute; Arlinghaus, Heinrich F.; Wiesmann, Hans‐Peter
- Abstract
In this study, time-of-flight secondary ion mass spectrometry (ToF-SIMS) and advanced electron microscopy (scanning electron microscopy (SEM), scanning transmission electron microscopy (STEM) and transmission electron microscopy (TEM)) were applied to detect and analyse different nano-scaled protein coatings on gold nanoparticles (NP). The NP were coated with collagen type I and fibronectin as well as different combinations of these proteins. These two main proteins in human cell organization and tissue formation were identified with the aid of ToF-SIMS by typical amino acid mass peak detection. In addition, the protein-coated particles were investigated by TEM and SEM to get information about the protein structure, the protein layer thickness on the particle surfaces and the reaction of NP in different protein solutions. In this study, a differentiation of diverse protein induced particle agglomeration was proven. The investigations of this study were part of the Specific Targeted Research Project CellNanoTox (project no. NMP4-CT-2006-032731) funded by the European Commission under the 6th EU Framework Programme for Research and Technological Development. Copyright © 2013 John Wiley & Sons, Ltd.
- Subjects
ELECTRON microscopy; SECONDARY ion mass spectrometry; GOLD nanoparticles; AMINO acids; CLUSTERING of particles; PROTEIN structure
- Publication
Surface & Interface Analysis: SIA, 2013, Vol 45, Issue 9, p1340
- ISSN
0142-2421
- Publication type
Article
- DOI
10.1002/sia.5287