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- Title
RNA Editing as a General Trait of Ebolaviruses.
- Authors
Mehedi, Masfique; Ricklefs, Stacy; Takada, Ayato; Sturdevant, Dan; Porcella, Stephen F; Marzi, Andrea; Feldmann, Heinz
- Abstract
RNA editing has been discovered as an essential mechanism for the transcription of the glycoprotein (GP) gene of Ebola virus but not Marburg virus. We developed a rapid transcript quantification assay (RTQA) to analyze RNA transcripts generated through RNA editing and used immunoblotting with a pan-ebolavirus monoclonal antibody to confirm different GP gene–derived products. RTQA successfully quantified GP gene transcripts during infection with representative members of 5 ebolavirus species. Immunoblotting verified expression of the soluble GP and the transmembrane GP. Our results defined RNA editing as a general trait of ebolaviruses. The degree of editing, however, varies among ebolaviruses with Reston virus showing the lowest and Bundibugyo virus the highest degree of editing.
- Subjects
RESTON (Va.); RNA editing; MARBURG virus; EBOLA virus; MONOCLONAL antibodies; IMMUNOBLOTTING
- Publication
Journal of Infectious Diseases, 2023, Vol 228, pS498
- ISSN
0022-1899
- Publication type
Article
- DOI
10.1093/infdis/jiad228