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- Title
Salivary HPV Persistence Following Treatment of Oropharyngeal Squamous Cell Carcinoma.
- Authors
Quimby, Alexandra E.; Lagiou, Pagona; Purgina, Bibiana; Corsten, Martin; Johnson-Obaseki, Stephanie
- Abstract
Objective: To determine the persistence of human papillomavirus (HPV) infection following treatment of HPV-positive oropharyngeal squamous cell carcinoma (HPV + OPSCC). Methods: A cross-sectional study was undertaken at The Ottawa Hospital (Ottawa, ON, Canada), a tertiary academic hospital and regional cancer center. Adult patients who were diagnosed with HPV + OPSCC between the years of 2014 and 2016 and treated with curative intent, and who were alive and willing to consent were eligible for inclusion. A saliva assay was used to test for the presence of HPV DNA in a random sample of patients. qPCR was used to amplify DNA from saliva samples. Results: Saliva samples were obtained from 69 patients previously treated with HPV + OPSCC. All patients had a minimum of 2 years of follow-up. 5 patients tested positive for HPV: 2 were positive for HPV-16, 2 for HPV-18, and 1 "other" HPV type. No patient in our study cohort had suffered recurrence post-treatment. Conclusions: This study is the first to demonstrate the prevalence of persistent oncogenic HPV DNA in saliva following treatment for HPV + OPSCC. This prevalence appears to be low, despite the fact that persistent HPV infection is a precursor for the development of HPV + OPSCC. This finding raises questions about what factors influence the clearance or persistence of HPV DNA in saliva after treatment for HPV + OPSCC, and may add to our understanding about the longitudinal effects of HPV infection in these cancers.
- Subjects
CANADA; DNA analysis; PAPILLOMAVIRUSES; RESEARCH; PILOT projects; REVERSE transcriptase polymerase chain reaction; ACADEMIC medical centers; SPECIALTY hospitals; SALIVA; CHRONIC diseases; CROSS-sectional method; MULTIPLE regression analysis; OROPHARYNGEAL cancer; CANCER relapse; INFECTION; CANCER patients; CANCER treatment; PAPILLOMAVIRUS diseases; CHI-squared test; DESCRIPTIVE statistics; DISEASE prevalence; STATISTICAL sampling; DATA analysis software; SQUAMOUS cell carcinoma; LONGITUDINAL method; PROPORTIONAL hazards models; DISEASE risk factors
- Publication
Annals of Otology, Rhinology & Laryngology, 2022, Vol 131, Issue 10, p1053
- ISSN
0003-4894
- Publication type
Article
- DOI
10.1177/00034894211055606