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- Title
Cloning and Characterization of a Novel Neuropeptide Y Receptor Subtype in the Zebrafish.
- Authors
LUNDELL, INGRID; BERGLUND, MAGNUS M.; STARBÄCK, PAULA; SALANECK, ERIK; GEHLERT, DONALD R.; LARHAMMAR, DAN
- Abstract
Neuropeptide Y (NPY), peptide YY (PYY), and pancreatic polypeptide (PP) form a family of structurally related peptides. As we have previously isolated clones for NPY and PYY from the zebrafish ( Danio rerio), we wished to clone the receptors for these peptides to allow correlation of ligand and receptor distribution. We describe here the cloning and functional expression of a receptor with equally high identity to the NPY-Y1 receptor as to the recently cloned Y4/PP1 and Y6 receptors with an overall amino acid sequence identity of approximately 50%. Furthermore, the zebrafish receptor gene lacks the intron present in the coding region in vertebrate Yl genes. These features strongly suggest that the zebrafish receptor represents a separate subtype. Hence, we have named it zYb for zebrafish Y-receptor b. (We have also discovered a unique receptor called zYa.) The zYb receptor has a binding profile that is reminiscent of Yl with affinities for NPY and PYY in the low picomolar range, whereas affinities for Y2-selective ligands are considerably lower. It couples to adenylyl cyclase by inhibiting cAMP synthesis. Receptor mRNA was detected by reverse transcription polymerase chain reaction (RT-PCR) in brain, eye, and intestine. The binding profile and amino acid identity show that the zebrafish zYb receptor is related to Yl but represents a distinct subtype that is likely to be present also in mammals.
- Publication
DNA & Cell Biology, 1997, Vol 16, Issue 11, p1357
- ISSN
1044-5498
- Publication type
Article
- DOI
10.1089/dna.1997.16.1357