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- Title
Epitope characterization of the protective monoclonal antibody VN04-2 shows broadly neutralizing activity against highly pathogenic H5N1.
- Authors
Lim, Angeline P. C.; Wong, Steven K. K.; Chan, Annie H. Y.; Chan, Conrad E. Z.; Eng Eong Ooi; Hanson, Brendon J.
- Abstract
The monoclonal antibody VN04-2 was previously shown to protect mice against lethal A/Vietnam/ 1203/04 H5N1 virus challenge when administered pre- and post-infection. In this study, we characterized the binding requirements of this antibody using direct binding to hemagglutinin and neutralization assays with H5N1 virus-like particles (H5N1-VLP) of eight recent H5N1 strains representing the major mutations within the 140s antigenic loop. Binding was clade independent and 3 mutations within this antigenic region are required before escape is possible, suggesting that apart from the H5N1 viruses circulating in Indonesia, VN04-2 may provide protection against H5N1 viruses from all other regions.
- Subjects
MONOCLONAL antibodies; AVIAN influenza; HEMAGGLUTININ; NEUTRALIZATION (Chemistry); GENETIC mutation; EPITOPES
- Publication
Virology Journal, 2008, Vol 5, p1
- ISSN
1743-422X
- Publication type
Article
- DOI
10.1186/1743-422X-5-80