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- Title
The long non-coding RNA lncMYOZ2 mediates an AHCY/MYOZ2 axis to promote adipogenic differentiation in porcine preadipocytes.
- Authors
Yang, Yang; Wu, Yiqi; Ji, Mengting; Rong, Xiaoyin; Zhang, Yanwei; Yang, Shuai; Lu, Chang; Cai, Chunbo; Gao, Pengfei; Guo, Xiaohong; Li, Bugao; Cao, Guoqing
- Abstract
Long non-coding RNAs (lncRNAs) play a vital role in regulating adipogenesis. However, the associated regulatory mechanisms have yet to be described in detail in pig. In this study, we demonstrate a critical role for lncMYOZ2 in adipogenesis from porcine preadipocytes. Specifically, lncMYOZ2 was more abundant in the adipose tissue of Mashen (fat-type) pigs than for Large White (lean-type) pigs, and knockdown of this lncRNA significantly inhibited the differentiation of porcine preadipocytes into adipocytes. Mechanistically, we used RNA pull-down and RIP assays to establish that lncMYOZ2 interacts with adenosylhomocysteinase (AHCY). Moreover, lncMYOZ2 knockdown increased promoter methylation of the target gene MYOZ2 and lowered its expression. Finally, we describe a positive regulatory role for MYOZ2 in adipogenesis. Collectively, these findings establish lncMYOZ2 as an important epigenetic regulator of adipogenesis via the aforementioned AHCY/MYOZ2 pathway, and provide insights into the role of lncRNAs in porcine adipose development.
- Subjects
NON-coding RNA; LINCRNA; ADIPOGENESIS; ADIPOSE tissues
- Publication
BMC Genomics, 2022, Vol 23, Issue 1, p1
- ISSN
1471-2164
- Publication type
Article
- DOI
10.1186/s12864-022-08923-9