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- Title
Identification of Dmrt genes and their up-regulation during gonad transformation in the swamp eel ( Monopterus albus).
- Authors
Sheng, Yue; Chen, Bo; Zhang, Liao; Luo, Majing; Cheng, Hanhua; Zhou, Rongjia
- Abstract
The swamp eel is a teleost fish with a characteristic of natural sex reversal and an ideal model for vertebrate sexual development. However, underlying molecular mechanisms are poorly understood. We report the identification of five DM ( doublesex and mab- 3) domain genes in the swamp eel that include Dmrt2, Dmrt2b, Dmrt3, Dmrt4 and Dmrt5, which encode putative proteins of 527, 373, 471, 420 and 448 amino acids, respectively. Phylogenetic tree showed that these genes are clustered into corresponding branches of the DM genes in vertebrates. Southern blot analysis indicated that the Dmrt1-Dmrt3-Dmrt2 genes are tightly linked in a conserved gene cluster. Notably, these Dmrt genes are up-regulated during gonad transformation. Furthermore, mRNA in situ hybridisation showed that Dmrt2, Dmrt3, Dmrt4 and Dmrt5 are expressed in developing germ cells. These results are evidence that the DM genes are involved in sexual differentiation in the swamp eel.
- Subjects
MONOPTERUS albus; GONADAL sex reversal; GENETIC regulation; FISHES; MOLECULAR biology; TRANSCRIPTION factors; FISH hybridization
- Publication
Molecular Biology Reports, 2014, Vol 41, Issue 3, p1237
- ISSN
0301-4851
- Publication type
Article
- DOI
10.1007/s11033-013-2968-6