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- Title
Maricaulisalexandrii sp. nov., a novel active bioflocculants-bearing and dimorphic prosthecate bacterium isolated from marine phycosphere.
- Authors
Zhang, Xiao-ling; Qi, Min; Li, Qiu-hong; Cui, Zhen-dong; Yang, Qiao
- Abstract
An aerobic, Gram-stain-negative, straight or curved rods and dimorphic prosthecate bacterium designated as strain LZ-16-1T was isolated from phycosphere microbiota of routinely laboratory-cultured and highly-toxic marine dinoflagellate Alexandrium catenella LZT09. Strain LZ-16-1T produces active bioflocculanting exopolysaccharides (EPS). Cells were dimorphic with non-motile prostheca, or non-stalked and motile by a single polar flagellum. Growth occurred at 10–40 °C, pH 5–9 and 1–8% (w/v) NaCl, with optimum growth at 25 °C, pH 7–8 in the presence of 2–4% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain LZ-16-1T was affiliated to the genus Maricaulis, and closely related to M. parjimensis MCS 25T (99.5%) and M. virginensis VC-5T (99.0%). However, based on genome sequencing and phylogenomic calculations, the average nucleotide identity (ANI) and digtal DNA-DNA genome hybridization (dDDH) values between strains LZ-16-1T and its closest relative, M. parjimensis MCS 25T were only 85.0 and 20.9%, respectively. The dominant fatty acids of strain LZ-16-1T were summed feature 8, C16:0, C17:0, C18:0, C18:1ω9c and summed feature 9. Major polar lipids were sulfoquinovosyl diacylglycerol, six glycolipids, one unidentified phospholipid and one unidentified polar lipid. The predominant isoprenoid quinone was Q-10. The DNA G + C content calculated from the genome was 63.6 mol%. Physiological and chemotaxonomic characterizations further confirmed the distinctiveness of strain LZ-16-1T from other Maricaulis members. Thus, strain LZ-16-1T represents a novel species of the genus Maricaulis, for which the name Maricaulis alexandrii sp. nov. (type strain LZ-16-1T = KCTC 72194T = CCTCC AB 2019006T) is proposed.
- Subjects
GLYCOLIPIDS; MARINE toxins; NUCLEOTIDE sequencing; SALT; FLAGELLA (Microbiology); BACTERIA; ALEXANDRIUM
- Publication
Antonie van Leeuwenhoek, 2021, Vol 114, Issue 8, p1195
- ISSN
0003-6072
- Publication type
Article
- DOI
10.1007/s10482-021-01588-6