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- Title
Effects of Leptin on Na<sup>+</sup>/Ca<sup>2+</sup> Exchanger in PC12 Cells.
- Authors
Zhang, Zijuan; Sun, Shuguang; Du, Caixia; Li, Wei; Zhang, Juan; Zhu, Yanqin; Liu, Peilin; Xing, Ying
- Abstract
Background/Aims: Alzheimer's disease (AD) is known to be related to alterations in neuronal intracellular calcium activity ([Ca2+]i). The present study revealed the distinct role of leptin in Na+/Ca2+-exchanger activity. Methods: [Ca2+]i was determined utilizing Fura-2 fluorescence. The activity of NCX was measured by removal of extracellular Na+ in the presence of external Ca2+. Na+/Ca2+-exchanger activity was further quantified from whole cell currents following removal of extracellular Na+. Na+/Ca2+-exchanger isoform NCX1 transcript levels and protein abundance were quantified by RT-PCR and Western blotting, respectively. Results: Exposure of PC12 cells to 30 μM amyloid (Aβ42) increased [Ca2+]i, an effect significantly blunted by 6 hours incubation with leptin before Aβ42 treatment. Moreover, leptin treatment significantly increased Na+/ Ca2+-exchanger mediated Ca+ transport and current, NCX1 transcript level as well as NCX1 membrane protein abundance. Conclusion: We show that leptin blunts Aβ42-evoked [Ca2+]i increase by increasing expression and activity of Na+/Ca2+-exchanger NCX1.
- Subjects
LEPTIN; PEPTIDE hormones; ALZHEIMER'S disease; INTRACELLULAR calcium; FLUORESCENCE
- Publication
Cellular Physiology & Biochemistry (Karger AG), 2016, Vol 40, Issue 6, p1529
- ISSN
1015-8987
- Publication type
Article
- DOI
10.1159/000453203