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- Title
Growth of Dunaliella tertiolecta and associated bacteria in photobioreactors.
- Authors
Lakaniemi, Aino-Maija; Intihar, Veera; Tuovinen, Olli; Puhakka, Jaakko
- Abstract
The aim of this study was to test three flat-plate photobioreactor configurations for cultivation of marine green alga Dunaliella tertiolecta under non-axenic growth conditions and to characterize and quantify the associated bacteria. The photobioreactor cultivations were conducted using tap water-based media. Static mixers intended to enhance mixing and light utilization did not generally increase algal growth at the low light intensities used. The maximum biomass concentration (measured as volatile suspended solids) and maximum specific growth rate achieved in the flat plate with no mixer were 2.9 g l and 1.3 day, respectively. Based on quantitative polymerase chain reaction, bacterial growth followed the growth of D. tertiolecta. Based on 16S rDNA amplification and denaturing gradient gel electrophoresis profiling, heterotrophic bacteria in the D. tertiolecta cultures mainly originated from the non-axenic algal inocula, and tap water heterotrophs were not enriched in high chloride media (3 % salinity). Bacterial communities were relatively stable and reproducible in all flat-plate cultivations and were dominated by Gammaproteobacteria, Flavobacteria, and Alphaproteobacteria.
- Subjects
DUNALIELLA tertiolecta; PHOTOBIOREACTORS; MICROALGAE; POLYMERASE chain reaction; RECOMBINANT DNA; GENE amplification; BIOMASS estimation
- Publication
Journal of Industrial Microbiology & Biotechnology, 2012, Vol 39, Issue 9, p1357
- ISSN
1367-5435
- Publication type
Article
- DOI
10.1007/s10295-012-1133-x