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- Title
采用木屑培养基传代的金针菇菌种稳定性.
- Authors
张丹; 朱爱莲; 赵静; 周敏; 张引芳; 尚晓冬; 王瑞娟; 谭琦
- Abstract
The stability of Flammulina velutipes spawn subcultured on a sawdust medium was studied. The original strain was subcultured for six consecutive passages and then each subculture was compared to the original starting strain in terms of mycelial growth rate, pH in liquid fermentation, agronomic traits of fruiting body pertaining to the factory production of F. velutipes, and genetic deviations at the DNA level. Compared to the original strain, all the six passages of strains maintained high stability in phenotypic traits, and genetic variations for the first five passages gradually increased as the number of subcultures increased. Compared to the original strain, genetic differences of the first four subcultures remained less than 4%, and the genetic difference of the fifth subculture was 7.92%. This experiment showed that the spawn quality of F. velutipes was effectively maintained on the sawdust medium.
- Publication
Acta Edulis Fungi, 2018, Vol 25, Issue 2, p29
- ISSN
1005-9873
- Publication type
Article
- DOI
10.16488/j.cnki.1005-9873.2018.02.003