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- Title
葉酸補充對高脂暴露人類肝癌細胞株 Hep3B 自噬 作用與脂質代謝分子表現之影響.
- Authors
李貞瑩; 許瑞芬
- Abstract
High fat leads to metabolic disorders and lipid accumulation in liver and hepatocarcinoma cell lines. Studies have reported the effect of autophagy on lipid degradation. It’s known that folic acid supplementation (FAS) regulate lipid metabolism, inflammation and other pathways to reduce fatty liver. Studies have shown that FAS downregulate autophagic markers in liver of high fat fed rats, but the mechanism is still unclear. The aim of this study was to investigate whether FAS on Hep3B improves high fat induced hepatic TG accumulation is regulated by autophagy. The study was designed to detect hepatocarcinoma cell lines Hep3B with similar human liver lipid metabolism. After FAS, 0.4mM oleic acid (OA) was given for 24 hours to induce TG accumulation, then detected autophagy markers. The results showed that (1) There’s no effect on 24H OA-exposed autophagy biomarkers. FAS reduce protein expression of (Beclin1) in OA-exposed Hep3B. (2) BafA1, an autolysosome inhibitor, reversed the effect of FAS, up-regulated autophagosome marker (LC3II), and more LC3 puncta was seen by immunofluorescence staining. (3) Oil red O staining found that FAS had no effect on 24H OA-exposed TG accumulation. Above all, it shows that FAS may downregulate early autophagy marker in OA-exposed Hep3B, but there’s no effects on TG accumulation caused by short-term high fat treatment, which needs to be further explored through long-term high fat treatment.
- Subjects
STAINS &; staining (Microscopy); FAS proteins; FOLIC acid; NON-alcoholic fatty liver disease; LIPID metabolism; FAT
- Publication
Nutritional Sciences Journal, 2023, Vol 47, Issue 1, p1
- ISSN
1011-6958
- Publication type
Article
- DOI
10.6691/NSJ.202303_47(1).0001