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- Title
逆境对 GST 基因过表达库德毕赤酵母 G43 生长及 脱镉能力的影.
- Authors
徐婉莹; 戚晓雪; 何晓霞; 徐 莹
- Abstract
Objective To explore the effects of stress on the growth and cadmium removal ability of Pichia kudriavzevii G43 overexpressed glutathione S-transferase (GST) gene. Methods The effects of salt, low pH, ethanol and high temperature on the growth and cadmium removal ability of recombinant P. kudriavzevii G43 overexpressed GST gene with high cadmium resistance and high cadmium removal rate were evaluated from 2 aspect of the biomass and cadmium removal rate. Results Stress culture conditions had great effects on the growth and cadmium removal ability of G43. Compared with the original strain P. kudriavzevii A16 (CK), the tolerances of G43 to salt, acid, ethanol and heat were significantly improved: G43 could tolerate 120 g/L NaCl, pH 2.5, 10% ethanol in the medium, or temperature of 43℃. Under different stress conditions, the cadmium removal capacity of G43 was equivalent to or significantly improved to that of the original strain. Conclusion The recombinant strain G43 has excellent tolerances, and different culture conditions have great influences on its cadmium removal effect, which provides basic data for the subsequent study of cadmium resistance and cadmium removal mechanism of P. kudriavzevii and other microorganisms, and also lays a theoretical foundation for its application in the field of cadmium removal in food system.
- Publication
Journal of Food Safety & Quality, 2022, Vol 13, Issue 11, p3620
- ISSN
2095-0381
- Publication type
Article