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- Title
Salt tolerance's toll: prolonged exposure to saline water inflicts damage to the blood cells of dice snakes (Natrix tessellata).
- Authors
Koleva, Vanya; Kornilev, Yurii; Telenchev, Ivan; Lukanov, Simeon; Hristova, Berna; Natchev, Nikolay
- Abstract
Non-invasive genetic sampling has provided valuable ecological data for many species - data which may have been unobtainable using invasive sampling methods. However, DNA obtained non-invasively may be prone to increased levels of amplification failure and genotyping error. Utilizing genotype data from 32 pedigreed koalas, this study aimed to validate the reliability of final consensus genotypes obtained using DNA isolated from koala scats. Pedigree analysis, duplicate genotyping, analysis of mismatched loci and tests for null alleles were used to look for evidence of errors. All genetically confirmed parent-offspring relationships were found to follow Mendelian rules of inheritance. Duplicate genotypes matched in all cases and there was no evidence of null alleles. Related individuals always had different 12-marker genotypes having a minimum of three unique loci (in one full sibling pair), a mode of seven unique loci and a maximum of 11 unique loci. This study demonstrates the capacity of DNA recovered from koala scats to provide reliable genotypes that can unequivocally discriminate individuals and infer parentage, provided data are missing from no more than two loci. Validating data obtained using non-invasive sampling is an important step, allowing potential problems to be identified at an early stage.
- Subjects
SALT industry; NATRIX tessellata; BLOOD cells; SALT springs; NATRIX
- Publication
Web Ecology, 2017, Vol 17, Issue 1, p1
- ISSN
2193-3081
- Publication type
Article
- DOI
10.5194/we-17-1-2017