We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Label-Free Optical Biosensing Using Low-Cost Electrospun Polymeric Nanofibers.
- Authors
Martínez-Pérez, Paula; Ponce-Alcántara, Salvador; Murillo, Nieves; Pérez-Márquez, Ana; Maudes, Jon; Peraile, Inés; González-López, Laura; Gil-García, Matilde; Lorenzo-Lozano, Paloma; García-Rupérez, Jaime
- Abstract
Polymeric nanofiber matrices are promising structures to develop biosensing devices due to their easy and affordable large-scale fabrication and their high surface-to-volume ratio. In this work, the suitability of a polyamide 6 nanofiber matrix for the development of a label-free and real-time Fabry–Pérot cavity-based optical biosensor was studied. For such aim, in-flow biofunctionalization of nanofibers with antibodies, bound through a protein A/G layer, and specific biodetection of 10 µg/mL bovine serum albumin (BSA) were carried out. Both processes were successfully monitored via reflectivity measurements in real-time without labels and their reproducibility was demonstrated when different polymeric nanofiber matrices from the same electrospinning batch were employed as transducers. These results demonstrate not only the suitability of correctly biofunctionalized polyamide 6 nanofiber matrices to be employed for real-time and label-free specific biodetection purposes, but also the potential of electrospinning technique to create affordable and easy-to-fabricate at large scale optical transducers with a reproducible performance.
- Subjects
NANOFIBERS manufacturing; OPTICAL transducers; NANOFIBERS; POLYAMIDES; SERUM albumin; CARRIER proteins; POROUS materials
- Publication
Chemosensors, 2020, Vol 8, Issue 4, p119
- ISSN
2227-9040
- Publication type
Article
- DOI
10.3390/chemosensors8040119