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- Title
Effects of Polygonatum cyrtonema Hua polysaccharide on C2C12 cells with cisplatin-induced myotube atrophy based on TMT proteomics.
- Authors
TANG Xueyang; ZHOU Rongrong; FANG Xiaoyang; HUANG Ao; ZENG Hongliang; XIE Jing; JIN Jian; ZHANG Shuihan
- Abstract
To investigate the protein metabolism profile of Polygonatum cyrtonema Hua polysaccharide (PCP) on cisplatin-induced myotube atrophy in mouse myoblasts (C2C12 cells) by tandem mass tag (TMT)-labeled quantitative proteomics, and to further explore the mechanism of PCP. Methods C2C12 cells were cultured into myotubes and divided into four groups: normal group, cisplatin group, PCP group, and cisplatin + PCP group. The cells in the normal group were administered serum-free high-glucose DMEM medium, the cells in the cisplatin group were administered serum-free high-glucose DMEM medium +50 μmol/L cisplatin, the cells in the PCP group were administered serumfree high-glucose DMEM medium+ 0. 200 mg/L PCP, the cells in the cisplatin + PCP group were administered serum-free high-glucose DMEM medium+50 μmol/L cisplatin +0. 200 mg/L PCP, and cells of all of the groups were cultured for 24 h in the dark. The viability of C2C12 cells was detected by the CCK8 method, the myotube diameter and cell fusion index were determined by an immunofluorescence method, and the protein metabolism profile of PCP that improved C2C12 myotube atrophy induced by cisplatin was characterized by TMT proteomics. Results CCK8 result showed that PCP promoted the viability of C2C12 cells, the modeling dose of cisplatin-induced C2C12 myotube atrophy was 50 μmol/L, and the mass concentration of PCP was 0. 200 mg/L. The result of immunofluorescence showed that, compared with the cisplatin group, the diameter and fusion index of the myotubes were increased after PCP intervention (P<0. 01). Proteomic result showed that 43 proteins, including phosphopantothenatecysteine ligase, pantothenate kinase 4, α-enolase and CCR4-NOT transcription complex subunit 9, underwent continuous regulatory changes among the normal group, cisplatin group, and cisplatin + PCP group. Regulation of metabolic pathways related to ribosomes, sphingolipid metabolism and sphingolipid biosynthesis contributed to the improvement of C2C12 myotube atrophy induced by cisplatin. Conclusion PCP improved C2C12 myotube atrophy induced by cisplatin, and its mechanism may be related to downregulating the accumulation of ceramide, which may be mediated by sphingomyelin phosphodiesterase 2.
- Subjects
CISPLATIN; POLYSACCHARIDES; SPHINGOMYELINASE; ATROPHY; PROTEOMICS; CELL fusion
- Publication
Journal of Beijing University of Traditional Chinese Medicine, 2023, Vol 46, Issue 6, p790
- ISSN
1006-2157
- Publication type
Article
- DOI
10.3969/j.issn.1006-2157.2023.06.009