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- Title
Gonadal expression profiles of sex-specific genes during early sexual differentiation in Japanese eel Anguilla japonica.
- Authors
Inaba, Hiroyuki; Hara, Seiji; Horiuchi, Moemi; Ijiri, Shigeho; Kitano, Takeshi
- Abstract
The Japanese eel Anguilla japonica is a prominent and highly valued species in the aquaculture industry in Japan. Sex determination and sex differentiation in eels are significantly affected by environmental factors; however, the molecular mechanisms involved in sex differentiation in eels are largely unknown. In this study, to investigate the gonadal expression profiles of sex-specific genes during and after sexual differentiation in Japanese eel, we induced elvers into predominantly phenotypic males or females by rearing on a control diet or estradiol-17β-treated diet, respectively, during the sex differentiation period. Quantitative real-time PCR showed that forkhead box L2A (foxl2a) and cytochrome P450 aromatase (cyp19a1) were more highly expressed in ovaries than in testes, whereas the expression levels of anti-Müllerian hormone (amh) and gonadal soma-derived factor (gsdf) were significantly higher in testes than in ovaries. Furthermore, foxl2a and cyp19a1 displayed female-specific expression early in the sex differentiation process, while after slightly more growth amh and gsdf displayed male-specific expression during sex differentiation. Together, these results suggest that these genes have important roles in sexual differentiation and development in this species.
- Subjects
JAPAN; ANGUILLA japonica; SEX differentiation (Embryology); GENE expression profiling; ANTI-Mullerian hormone; CYTOCHROME P-450
- Publication
Fisheries Science, 2021, Vol 87, Issue 2, p203
- ISSN
0919-9268
- Publication type
Article
- DOI
10.1007/s12562-020-01491-5