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- Title
Serum antibodies and DNA indicate a high prevalence of equine papillomavirus 2 (Ec PV2) among horses in Switzerland.
- Authors
Fischer, Nina. M.; Favrot, Claude; Birkmann, Katharina; Jackson, Michele; Schwarzwald, Colin C.; Müller, Martin; Tobler, Kurt; Geisseler, Marco; Lange, Christian E.
- Abstract
Background The DNA of equine papillomavirus type 2 ( Ec PV2) is consistently found in equine papillomas and squamous cell carcinomas, indicating a causal association of Ec PV2 in the pathogenesis of these tumours; however, little is known about the prevalence of this virus. Hypothesis/Objectives The aim of this study was to determine the geno- and seroprevalence of Ec PV2 in clinically healthy horses in Switzerland. Animals Fifty horses presented to the equine department of the university clinic, displaying no skin or mucous membrane lesions or severe signs of other diseases, were sampled. Methods Cytobrush samples from the penis or vulva and serum samples were collected. To determine the genoprevalence of Ec PV2, DNA was extracted from cytobrush samples and tested for viral DNA with a PCR assay amplifying a 338 bp fragment of the E7/ E1 region of the viral genome. Seroprevalence was tested using an enzyme-linked immunosorbent assay aimed to detect antibodies against the major capsid protein ( L1) of Ec PV2. Results In five of 50 horses (10%), Ec PV2-specific DNA was amplified but no antibodies could be detected, whereas in 14 of 50 horses (28%), antibodies against Ec PV2 but no DNA were demonstrated. Both antibodies and viral DNA were detected in four of 50 horses (8%). Neither antibodies nor viral DNA were found in 27 of 50 horses (54%). Conclusions and clinical importance The seroprevalence suggests that Ec PV2 is prevalent in the Swiss equine population, while the genoprevalence indicates that currently ongoing infections are less common. The discrepancy between geno- and seroprevalence probably indicates different stages of infection in the tested cohort.
- Subjects
SWITZERLAND; IMMUNOGLOBULINS; DNA; HORSE diseases; PAPILLOMAVIRUS diseases
- Publication
Veterinary Dermatology, 2014, Vol 25, Issue 3, p210
- ISSN
0959-4493
- Publication type
Article
- DOI
10.1111/vde.12129