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- Title
Novel chicken two-dimensional intestinal model comprising all key epithelial cell types and a mesenchymal sub-layer.
- Authors
Orr, Brigid; Sutton, Kate; Christian, Sonja; Nash, Tessa; Niemann, Helle; Hansen, Lone Lind; McGrew, Mike J.; Jensen, Stina Rikke; Vervelde, Lonneke
- Abstract
The intestinal epithelium plays a variety of roles including providing an effective physical barrier and innate immune protection against infection. Two-dimensional models of the intestinal epithelium, 2D enteroids, are a valuable resource to investigate intestinal cell biology and innate immune functions and are suitable for high throughput studies of paracellular transport and epithelial integrity. We have developed a chicken 2D enteroid model that recapitulates all major differentiated cell lineages, including enterocytes, Paneth cells, Goblet cells, enteroendocrine cells and leukocytes, and self-organises into an epithelial and mesenchymal sub-layer. Functional studies demonstrated the 2D enteroids formed a tight cell layer with minimal paracellular flux and a robust epithelial integrity, which was maintained or rescued following damage. The 2D enteroids were also able to demonstrate appropriate innate immune responses following exposure to bacterial endotoxins, from Salmonella enterica serotype Typhimurium and Bacillus subtilis. Frozen 2D enteroids cells when thawed were comparable to freshly isolated cells. The chicken 2D enteroids provide a useful ex vivo model to study intestinal cell biology and innate immune function, and have potential uses in screening of nutritional supplements, pharmaceuticals, and bioactive compounds.
- Subjects
EPITHELIAL cells; SALMONELLA enterica serovar typhimurium; TWO-dimensional models; ENTEROENDOCRINE cells; SALMONELLA typhimurium; CYTOLOGY; INTESTINAL mucosa; MUCUS
- Publication
Veterinary Research, 2021, Vol 52, Issue 1, p1
- ISSN
0928-4249
- Publication type
Article
- DOI
10.1186/s13567-021-01010-z