We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Innovative hydroxyapatite-based coatings for bone implants: A multifaceted approach.
- Authors
Stevanović, Milena; Đošić, Marija; Janković, Ana; Sprecher, Christoph Martin; Vukašinović-Sekulić, Maja; Mišković-Stanković, Vesna
- Abstract
INTRODUCTION: Tissue engineering strives for innovative solutions in addressing challenges associated with contemporary bone tissue implants. This study focuses on the electrophoretic deposition (EPD) of hydroxyapatite-based bioceramic composites containing antibacterial agents onto titanium surfaces. Two composite coatings, hydroxyapatite/chitosan (HAP/CS) and hydroxyapatite/chitosan/gentamicin (HAP/CS/Gent) were developed to combat issues such as poor adhesion, limited antibacterial potential, limited bioactivity, and potential toxicity of implant materials. EXPERIMENTAL: EPD was performed at constant voltage (5 V, 12 min) on pure Ti plates from aqueous (HAP/CS and HAP/CS/Gent) suspensions. The uniformity and functionality of the deposited coatings were assessed through comprehensive physico-chemical characterization using X-ray diffraction (XRD) (Philips PW 1710, Netherlands) and scanning electron microscopy (SEM, Hitachi S-4700, J) equipped with energy dispersive X-Ray spectroscopy (EDS, X-Max, Oxford Instruments, UK). Antibacterial activity was evaluated against Staphylococcus aureus TL and Escherichia coli ATCC 25922 by quantitatively monitoring changes in the viable number of bacterial cells in suspension. Cytotoxicity against MRC-5 and L929 cell lines was investigated using trypan blue dye-exclusion test (DET) and MTT assay for assessing cell metabolic activity. Statistical significance was determined for antibacterial and cytotoxicity results by one-way analysis of variance (ANOVA), followed by multiple comparisons post-hoc test. RESULTS AND DISCUSSION: XRD revealed broadened diffraction maximums corresponding to fine HAP crystallites. Porous surface with homogeneously distributed spherical HAP agglomerates embedded in wax-like polymers' matrix of CS was observed for both coatings by SEM. The addition of gentamicin significantly enhanced the antibacterial activity of the HAP/CS/Gent coating – complete reduction of S. aureus bacterial cells was achieved within 1 h of exposure. MTT and DET tests indicated low cytotoxicity against MRC-5 and L929 tested cell line for both samples. Slightly decreased cell percentage viability due to gentamicin presence was observed for HAP/CS/Gent. CONCLUSIONS: Single-step EPD yielded antibacterial composite coatings with potential for biomedical applications. HAP/CS/Gent showed successful gentamicin loading, favorable crystalline structure, and strong antibacterial effects. The presence of fine HAP crystallites yielded a larger surface area, favorable for new bone growth and improved osseointegration. HAP/CS/Gent exhibited good antibacterial activity against both tested bacteria (especially pronounced against S. aureus – bactericidal effect), while preserving low cytotoxicity, indicating the high potential for biomedical applications.
- Subjects
NETHERLANDS; HYDROXYAPATITE coating; HYDROXYAPATITE; OXFORD Instruments PLC; ENERGY dispersive X-ray spectroscopy; COMPOSITE coating; ONE-way analysis of variance; SURFACE coatings; CYTOTOXINS
- Publication
Chemical Industry / Hemijska Industrija, 2024, Vol 78, p58
- ISSN
0367-598X
- Publication type
Article