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- Title
Improvement of Tol2 Transposon System by Modification of Tol2 Transposase.
- Authors
Park, Ji Yun; Lee, Haneur; Song, Eun Seon; Lee, Yun Haeng; Kuk, Myeong Uk; Ko, Gahyun; Byun, Youngjoo; Kwon, Hyung Wook; Park, Joon Tae
- Abstract
Tol2 transposon-based vector platform has been developed as a useful strategy to improve protein yield. Tol2 transposase identifies inverted terminal repeats of transposons and enhances transgene integration efficiency in the genome. However, Tol2 transposon system does not reach a level of efficiency for use in commercial production, limiting its usefulness for mass production of recombinant proteins. Here, we proposed a novel strategy to improve the existing Tol2 transposase by adding a nuclear localization signal of histone H2B next to the Tol2 transposase (i.e., NLS-H2B-Tol2 transposase). Comparing the NLS-H2B-Tol2 transposase with the traditional Tol2 transposase, a significant increase in protein production was measured. Concurrently, a significant increase in nuclear transport of NLS-H2B-Tol2 transposase was observed, suggesting a causal relationship between increased nuclear transport and increased protein production. Furthermore, importin beta maximized NLS-H2B-Tol2-mediated protein productivity. Taken together, our findings offer useful ways for improving conventional Tol2 transposon-based vector platform. This new platform will be a breakthrough in the field of biopharmaceuticals to produce therapeutic proteins.
- Subjects
TRANSPOSONS; CARRIER proteins; RECOMBINANT proteins; MASS production; PROTEIN transport; BIOPHARMACEUTICS
- Publication
Biotechnology & Bioprocess Engineering, 2022, Vol 27, Issue 6, p987
- ISSN
1226-8372
- Publication type
Article
- DOI
10.1007/s12257-022-0175-9