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- Title
DNA Fingerprinting of Vegetable Soybean Cultivar 'Zhexian No.9' using 101 New Developed HRM-Based SNP Markers.
- Authors
Fu, X. J.; Pei, J. X.; Zheng, Y. T.; Guo, D. D.; Yang, Q. H.; Jin, H. X.; Zhu, D. H.; Dong, D. K.; Xu, S. C.
- Abstract
Single nucleotide polymorphisms (SNPs) have been proved to be powerful markers in genetic analysis due to their high abundance and polymorphism in plant genomes. The recently developed high-resolution melting (HRM) analysis method provides a novel, quick, and close-tube PCR approach to analyze SNP variations. In present study, 101 HRM-based SNP markers from 20 soybean chromosomes were developed for genotyping vegetable soybean cultivar 'Zhexian No.9' with 'Williams 82' as reference. 33.7% of these markers were polymorphic between 'Zhexian No.9' and 'Williams 82'. Polymorphic markers were found on 85% (17 of 20) of the soybean chromosomes when comparing 'Zhexian No.9' and 'Williams 82'. Finally, an array of 101 in-sequence nucleotide letters was generated as the first precise SNP fingerprint of 'Zhexian No.9'. The described marker-developing methodology could be used in other crops with known genomic information.
- Subjects
SOYBEAN; SINGLE nucleotide polymorphisms; DNA fingerprinting; PLANT genomes; GENETIC markers; CHROMOSOMES
- Publication
Legume Research: An International Journal, 2020, Vol 43, Issue 1, p8
- ISSN
0250-5371
- Publication type
Article
- DOI
10.18805/LR-456