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- Title
Sequential process in brain-derived neurotrophic factor-induced functional periodontal tissue regeneration.
- Authors
Konishi, Akihiro; Takeda, Katsuhiro; Fujita, Tsuyoshi; Kajiya, Mikihito; Matsuda, Shinji; Kittaka, Mizuho; Shiba, Hideki; Kurihara, Hidemi
- Abstract
We recently demonstrated that brain-derived neurotrophic factor ( BDNF) promotes periodontal tissue regeneration. The purpose of this study was to establish an essential component of a rational approach for the clinical application of BDNF in periodontal regenerative therapy. Here, we assessed the sequence of early events in BDNF-induced periodontal tissue regeneration, especially from the aspect of cementum regeneration. Brain-derived neurotrophic factor was applied into experimental periodontal defects in Beagle dogs. The localization of cells positive for neurotrophic tyrosine kinase, receptor, type 2, proliferating cell nuclear antigen, osteopontin, integrin αV β3, and integrin α2 β1 was evaluated by immunohistochemistry. The effects of BDNF on adhesion of cultured human periodontal ligament cells was examined by an in vitro study. The results suggest that BDNF could induce rapid cementum regeneration by stimulating adhesion, proliferation, and differentiation of periodontal ligament cells in the early regenerative phase, resulting in enhancement of periodontal tissue regeneration.
- Subjects
ANIMAL experimentation; CELL culture; CELLS; DOGS; GUIDED tissue regeneration; IMMUNOBLOTTING; IMMUNOHISTOCHEMISTRY; PERIODONTAL ligament; POLYMERASE chain reaction; RESEARCH funding; SCANNING electron microscopy; BRAIN-derived neurotrophic factor
- Publication
European Journal of Oral Sciences, 2016, Vol 124, Issue 2, p141
- ISSN
0909-8836
- Publication type
Article
- DOI
10.1111/eos.12244