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- Title
Immunolocalization of V<sub>1</sub> vasopressin receptors in the rat kidney using anti-receptor antibodies.
- Authors
Gonzalez, Carlos B.; Figueroa, Carlos D.; Reyes, Carlos E.; Caorsi, Carlos E.; Troncoso, Silvia; Menzel, Doris
- Abstract
Immunolocalization of V1 vasopressin receptors in the rat kidney using anti-receptor antibodies. By using immunocytochemical techniques we have been able to localize the V1 vasopressin receptor in the rat kidney, Immunoblotting using an antiserum raised against an affinity-purified vasopressin receptor showed a 55,000 daltons protein band that bas a molecular mass similar to that of the liver V1 vasopressin receptor, as demonstrated by cross-linking studies. Immunoblotting of the antibody showed a band of 55,000 daltons in A-10 cells, which contains the V1 subtype, whereas it did not stain LLC-PK1 cells, which possess the V2. subtype, showing that the antibody recognizes the V, vasopressin receptor. The immunostaining of kidney sections with this antiserum showed a strong reaction of the connecting tubules and cortical and medullary collecting ducts. The immunostaining pattern of connecting tubule and collecting duct cells was different, that is, the former showed a staining of both the apical and basal plasma membrane but also in the cytoplasm, whereas the latter showed a strong reaction mainly in the basolateral membrane. Immunostaining of consecutive serial sections with an antiserum raised against tissue kallikrein, an enzyme present exclusively in connecting tubules, and with the anti-receptor serum allowed us to show, for the first time, the presence of the vasopressin receptor in the connecting tubule cells and their absence in intercalated cells, the other cell type present in connecting tubules. These findings support experiments carried in the eighties on the release of renal tissue kallikrein by AVP.
- Subjects
PITUITARY hormones; VASOPRESSIN; LABORATORY rats; IMMUNOCYTOCHEMISTRY; IMMUNOFLUORESCENCE; KALLIKREIN; PANCREATIC secretions
- Publication
Kidney International, 1997, Vol 52, Issue 5, p1206
- ISSN
0085-2538
- Publication type
Article
- DOI
10.1038/ki.1997.445