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- Title
A rapid and efficient single-cell manipulation method for screening antigen-specific antibody–secreting cells from human peripheral blood.
- Authors
Jin, Aishun; Ozawa, Tatsuhiko; Tajiri, Kazuto; Obata, Tsutomu; Kondo, Sachiko; Kinoshita, Koshi; Kadowaki, Shinichi; Takahashi, Kazuo; Sugiyama, Toshiro; Kishi, Hiroyuki; Muraguchi, Atsushi
- Abstract
Antigen-specific human monoclonal antibodies (mAbs) are key candidates for therapeutic agents. However, the availability of a suitable screening system for antigen-specific antibody–secreting cells (ASCs) is limited in humans. Here we present a unique method for detecting individual ASCs using microwell array chips, which enables the analysis of live cells on a single-cell basis and offers a rapid, efficient and high-throughput (up to 234,000 individual cells) system for identifying and recovering objective ASCs. We applied the system to detect and retrieve ASCs for hepatitis B virus and influenza viruses from human peripheral blood lymphocytes and produced human mAbs with virus-neutralizing activities within a week. Furthermore, we show that the system is useful for detecting ASCs for multiple antigens as well as for selection of ASCs secreting high-affinity antibodies on a chip. Our method can open the way for the generation of therapeutic antibodies for individual patients.
- Subjects
MONOCLONAL antibodies; HEPATITIS B virus; INFLUENZA viruses; LYMPHOCYTES; ANTIGENS
- Publication
Nature Medicine, 2009, Vol 15, Issue 9, p1088
- ISSN
1078-8956
- Publication type
Article
- DOI
10.1038/nm.1966