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- Title
Immunohistochemical localisation of aquaporin 2 and vasopressin type 2 receptor in the human endolymphatic sac.
- Authors
Pan, X; Huang, C; Peng, A; Zhang, Z
- Abstract
Objective: This study aimed to determine the distribution and subcellular localisation of aquaporin 2 and vasopressin type 2 receptor in the human endolymphatic sac. Methods: Ten samples of human endolymphatic sac were collected during acoustic neurinoma removal using the translabyrinthine approach. Immunohistochemistry and immunofluorescence were performed using aquaporin 2 and vasopressin type 2 receptor monoclonal antibodies. Results: Confocal microscopy demonstrated that vasopressin type 2 receptor labelling was expressed in both the apical and basolateral plasma membranes, and in the cytoplasm of the endolymphatic sac epithelium, whereas aquaporin 2 was strongly expressed at the basolateral site of the endolymphatic sac epithelium, in both the intraosseous and extraosseous parts of the endolymphatic sac. Conclusion: Both aquaporin 2 and vasopressin type 2 receptor were detected in the epithelial cells of the human endolymphatic sac, suggesting that this channel may be involved in inner-ear fluid homeostasis. However, strong basolateral expression of aquaporin 2 in endolymphatic sac epithelium suggested that the function of aquaporin 2 may differ between the endolymphatic sac and kidney.
- Subjects
HOMEOSTASIS; IN vitro studies; STAINS &; staining (Microscopy); IMMUNOHISTOCHEMISTRY; MICROSCOPY; CELL receptors; FLUORESCENT antibody technique; MEMBRANE proteins; EPITHELIAL cells; VASOPRESSIN; COLLECTION &; preservation of biological specimens; ACOUSTIC neuroma; ENDOLYMPHATIC sac; CYTOPLASM
- Publication
Journal of Laryngology & Otology, 2023, Vol 137, Issue 12, p1340
- ISSN
0022-2151
- Publication type
Article
- DOI
10.1017/S0022215122002444