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- Title
PPAR-α agonism improves whole body and muscle mitochondrial fat oxidation, but does not alter intracellular fat concentrations in burn trauma children.
- Authors
Cree, Melanie G.; Newcomer, Bradley R.; Herndon, David N.; Ting Qian; Dayoung Sun; Morio, Beatrice; Zwetsloot, Jennifer J.; Dohm, G. Lynis; Fram, Ricki Y.; Aarsland, Asle; Wolfe, Robert R.
- Abstract
Insulin resistance is often associated with increased levels of intracellular triglycerides, diacylglycerol and decreased fat β-oxidation. It was unknown if this relationship was present in patients with acute trauma induced insulin resistance. A double blind placebo controlled trial was conducted in 18 children with severe burn injury. Metabolic studies to assess whole body palmitate oxidation and insulin sensitivity, muscle biopsies for mitochondrial palmitate oxidation, diacylglycerol, fatty acyl Co-A and carnitine concentrations, and magnetic resonance spectroscopy for muscle and liver triglycerides were compared before and after two weeks of placebo or PPAR-α agonist treatment. Insulin sensitivity and basal whole body palmitate oxidation as measured with an isotope tracer increased significantly (P=0.003 and P=0.004, respectively) after PPAR-α agonist treatment compared to placebo. Mitochondrial palmitate oxidation rates in muscle samples increased significantly after PPAR-α treatment (P=0.002). However, the concentrations of muscle triglyceride, diacylglycerol, fatty acyl CoA and carnitine and liver triglycerides did not change with either treatment. PKC-θ activation during hyper-insulinemia decreased significantly following PPAR-α treatment. Impaired palmitate oxidation and increased PKC activity are related to reduced insulin sensitivity in acute trauma, but a relation between these two responses cannot be attributed to alterations in intracellular lipid concentrations.
- Subjects
PEROXISOMES; FAT; OXIDATION; BURNS &; scalds in children; CHILDREN'S injuries; INSULIN resistance; INSULIN
- Publication
FASEB Journal, 2007, Vol 21, Issue 6, pA839
- ISSN
0892-6638
- Publication type
Article
- DOI
10.1096/fasebj.21.6.a839