We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
A Functional Mutation in KIAA1462 Promoter Decreases Glucocorticoid Receptor Affinity and Affects Egg-Laying Performance in Yangzhou Geese.
- Authors
Xia, Mengyuan; Wei, Wei; Jiang, Zaohang; He, Dandan; Li, Zhen; Yu, Shigang; Wang, Qiushi; Liu, Honglin; Chen, Jie
- Abstract
The identification of genetic markers is valuable for improving the egg-laying performance in goose production. The single-nucleotide polymorphism (SNP) rs1714766362 in an intron of the goose <italic>KIAA1462</italic> gene was found to be relevant to laying performance in our previous study. However, its function remains unclear. In this study, the full-length coding sequence of <italic>KIAA1462</italic> gene was firstly characterized in Yangzhou geese. Q-PCR (Quantitative Real Time Polymerase Chain Reaction) results showed that <italic>KIAA1462</italic> was highly expressed in the liver, ovary, and mature F1 follicles. For SNP rs1714766362, geese with the AA genotype showed better laying performance than the TT ones and exhibited a higher <italic>KIAA1462</italic> expression level in the ovary. Gain- and loss-of function experiments in granulosa cells revealed that <italic>KIAA1462</italic> affected the expression of the apoptosis marker gene caspase-3. Considering that rs1714766362 locates in an intron area, we compared the <italic>KIAA1462</italic> promoter regions of AA and TT individuals and identified the SNP c.-413C>G (Genbank ss2137504176), which was completely linked to SNP rs1714766362. According to the transcription factor prediction results, the glucocorticoid receptor (GR) would bind to the SNP site containing the C but not the G allele. In this study, we proved this hypothesis by an electrophoretic mobility shift assay (EMSA). In summary, we identified a novel mutation in the promoter of <italic>KIAA1462</italic> gene which can modulate GR binding affinity and affect the laying performance of geese.
- Subjects
GLUCOCORTICOID receptors; GEESE; MONOTREMES; POLYMERASE chain reaction; CASPASE genetics
- Publication
International Journal of Molecular Sciences, 2018, Vol 19, Issue 5, p1531
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms19051531