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- Title
Relación de los niveles séricos de IL-32 y del polimorfismo rs45499297 con la obesidad en pacientes mexicanos: un análisis de laboratorio e in silico.
- Authors
Andrea Martínez-Pérez, Luz; Sarai Becerra-Ruiz, Julieta; Esther García-Aviña, Juana; Denisse González-Sánchez, Grecia; Martínez-Esquivias, Fernando; Isela Vázquez-Jiménez, Sonia; los Santos, Saúl Ramírez-De; Iván López-Pulido, Édgar; Guzmán-Flores, Juan Manuel; Martínez Pérez, Luz Andrea; Becerra-Ruiz, Julieta Sarai; García-Aviña, Juana Esther; González-Sánchez, Grecia Denisse; Vázquez-Jiménez, Sonia Isela; Ramírez-De Los Santos, Saúl; López-Pulido, Edgar Iván
- Abstract
<bold>Introduction: </bold>Background: many genes have been involved in the development of obesity. Interleukin 32 (IL-32) is a proinflammatory cytokine; rs45499297 is a T/C promoter, single-nucleotide polymorphism of the IL32 gene. Objectives: this study aimed to evaluate the rs45499297 polymorphism and its association with obesity. Another objective of this study was to carry out an in silico analysis. Methods: this study was cross-sectional, and included 333 subjects classified by body mass index and fat percentage. The plasma glucose and lipid profile were measured. We measured serum IL-32 protein by ELISA and the rs45499297 polymorphism by PCR-RFLP. We used several databases to build the IL32 gene network and infer transcription factors that bind to this polymorphic site. Results: subjects underweight and with low fat percentages had lower levels of IL-32. CT genotype and allele C were less frequent in the overweight/obesity group than in the normal-weight group. Interestingly, this result remained only in the male gender. We found that the transcription factors Hepatocyte Nuclear Factor and Specificity Protein 1 bind to this polymorphic site. In addition, we infer that IL32 is involved in metabolic pathways related to viral infections. Conclusion: the TC genotype is associated with overweight/obesity. The decrease in levels of IL-32 observed in underweight and low fat percentage groups could be due to an impaired inflammatory profile. The in silico analysis showed that several transcriptional factors bind at this polymorphic site, and that the enrichment of the metabolic pathways is diverse.
- Subjects
OBESITY; INTERLEUKINS; CROSS-sectional method; GENETIC polymorphisms; DISEASE susceptibility; GENOTYPES
- Publication
Nutrición Hospitalaria, 2022, Vol 39, Issue 2, p313
- ISSN
0212-1611
- Publication type
Article
- DOI
10.20960/nh.03804