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- Title
Synovial inflammation plays a greater role in post-traumatic osteoarthritis compared to idiopathic osteoarthritis in the Hartley guinea pig knee.
- Authors
Thomas, Nathan P.; Wu, Wesley J.; Fleming, Braden C.; Fangyuan Wei; Qian Chen; Lei Wei; Wei, Fangyuan; Chen, Qian; Wei, Lei
- Abstract
<bold>Background: </bold>The objective of this study was to evaluate the extent of stromal cell-derived factor-1's (SDF-1) involvement in the pathogenesis of idiopathic versus post-traumatic OA by comparing differences in synovial membrane morphology, SDF-1 synovial fluid (SF) concentrations, and matrix metalloproteinase-13 (MMP-13) SF concentrations.<bold>Methods: </bold>Thirty-six 3-month-old Hartley guinea pigs were obtained and divided into 6 groups. Upon sacrifice, India Ink staining was used to evaluate gross morphology, Safranin O/Fast green staining was used to assess cartilage damage, H/E staining was employed to visualize the synovium, and SF samples were obtained for biochemical analyses. Sandwich ELISA was used to quantify the SF concentrations of SDF-1 and MMP-13.<bold>Results: </bold>12 month-old, idiopathic OA guinea pigs and 5.5 month-old ACLT animals had comparable cartilage damage when evaluated by the Modified Mankin Score. SDF-1 and MMP-13 concentrations were not statistically different between the two groups. The synovial membrane of the 5.5 month ACLT group had severe synovitis compared to the idiopathic OA group.<bold>Conclusion: </bold>In this study, it was found that synovial inflammation, independent of cartilage morphology, SDF-1 concentration, and MMP-13 concentration, was markedly different between idiopathic and post-traumatic OA. These results highlight the differing morphological and biochemical profiles of post-traumatic versus idiopathic osteoarthritis and calls for a more thorough examination of the sole of the synovial membrane in the pathogenesis of post-traumatic osteoarthritis.
- Subjects
OSTEOARTHRITIS; SYNOVIAL fluid; STROMAL cells; MATRIX metalloproteinases; GUINEA pigs as laboratory animals
- Publication
BMC Musculoskeletal Disorders, 2017, p1
- ISSN
1471-2474
- Publication type
journal article
- DOI
10.1186/s12891-017-1913-6