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- Title
GABAA receptor subunit β1 is involved in the formation of protease-resistant prion protein in prion-infected neuroblastoma cells
- Authors
Kimura, Tomohiro; Ishikawa, Kensuke; Sakasegawa, Yuji; Teruya, Kenta; Sata, Tetsutaro; Schätzl, Hermann; Doh-ura, Katsumi
- Abstract
Abstract: γ-Aminobutyric acid type A (GABAA) receptor β1 (gabrb1), a subunit of GABAA receptors involved in inhibitory effects on neurotransmission, was found to associate with the formation of protease-resistant prion protein in prion-infected neuroblastoma cells. Silencing of gabrb1 gene expression significantly decreased the abnormal prion protein level but paradoxically increased the normal prion protein level. Treatment with a gabrb1-specific inhibitor, salicylidene salicylhydrazide, dose-dependently decreased the abnormal prion protein level, but silencing of other GABAA receptor subunits’ gene expression and treatments with the receptor antagonists and agonists did not. Therefore, gabrb1 involvement in abnormal prion protein formation is independent of GABAA receptors.
- Subjects
GABA receptors; PROTEOLYTIC enzymes; PRION diseases; NEUROBLASTOMA; NEURAL transmission; GENE expression; NON-coding RNA
- Publication
FEBS Letters, 2010, Vol 584, Issue 6, p1193
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2010.02.029