We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
The cell cycle regulator p27<sup>Kip1</sup> interacts with MCM7, a DNA replication licensing factor, to inhibit initiation of DNA replication
- Authors
Nallamshetty, Shriram; Crook, Martin; Boehm, Manfred; Yoshimoto, Takanobu; Olive, Michelle; Nabel, Elizabeth G.
- Abstract
Abstract: The G1/S phase restriction point is a critical checkpoint that interfaces between the cell cycle regulatory machinery and DNA replicator proteins. Here, we report a novel function for the cyclin-dependent kinase inhibitor p27Kip1 in inhibiting DNA replication through its interaction with MCM7, a DNA replication protein that is essential for initiation of DNA replication and maintenance of genomic integrity. We find that p27Kip1 binds the conserved minichromosome maintenance (MCM) domain of MCM7. The proteins interact endogenously in vivo in a growth factor-dependent manner, such that the carboxyl terminal domain of p27Kip1 inhibits DNA replication independent of its function as a cyclin-dependent kinase inhibitor. This novel function of p27Kip1 may prevent inappropriate initiation of DNA replication prior to S phase.
- Subjects
NUCLEIC acids; DNA replication; PROTEIN kinases; GEL electrophoresis; SERUM albumin; CYTOKINES
- Publication
FEBS Letters, 2005, Vol 579, Issue 29, p6529
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2005.10.028