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- Title
Induction of 1,N<sup>2</sup>-etheno-2′-deoxyguanosine in DNA exposed to β-carotene oxidation products
- Authors
Marques, Sabrina A.; Loureiro, Ana Paula M.; Gomes, Osmar F.; Garcia, Camila C.M.; Di Mascio, Paolo; Medeiros, Marisa H.G.
- Abstract
Epidemiological studies testing the effect of β-carotene in humans have found a relative risk for lung cancer in smokers supplemented with β-carotene. We investigated the reactions of retinal and β-apo-8′-carotenal, two β-carotene oxidation products, with 2′-deoxyguanosine to evaluate their DNA damaging potential. A known mutagenic adduct, 1,N2-etheno-2′-deoxyguanosine, was isolated and characterized on the basis of its spectroscopic features. After treatment of calf thymus DNA with β-carotene or β-carotene oxidation products, significantly increased levels of 1,N2-etheno-2′-deoxyguanosine and 8-oxo-7,8-dihydro-2′-deoxyguanosine were quantified in DNA. These lesions are believed to be important in the development of human cancers. The results reported here may contribute toward an understanding of the biological effects of β-carotene oxidation products.
- Subjects
CAROTENES; DNA damage; MASS spectrometry; LIQUID chromatography
- Publication
FEBS Letters, 2004, Vol 560, Issue 1-3, p125
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/S0014-5793(04)00084-5