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- Title
Overexpression of Organellar and Cytosolic AtHSP90 in Arabidopsis thaliana Impairs Plant Tolerance to Oxidative Stress.
- Authors
Hongmiao Song; Pengxiang Fan; Yinxin Li
- Abstract
Three AtHSP90 isoforms, cytosol-localized AtHSP90.2, chloroplast-localized AtHSP90.5, and endoplasmic reticulum (ER)-localized AtHSP90.7 genes, were constitutively overexpressed in Arabidopsis thaliana to study their functional mechanisms under oxidative stress. Overexpression of AtHSP90 genes reduced germination of transgenic seeds under oxidative stress. When exposed to 10 mM H2O2, AtHSP90 transgenic seedlings displayed lower activities of superoxide dismutase, catalase, and peroxidase; higher content of malondialdehyde; and higher levels of protein damage than detected in the wild type. This indicated that overexpression of AtHSP90.2, AtHSP90.5, and AtHSP90.7 in Arabidopsis impaired plant tolerance to oxidative stress. Moreover, overexpression of chloroplast- and ER-localized AtHSP90 resulted in lower resistance to oxidative stress than that of cytosolic AtHSP90. This suggested that HSP90.2, HSP90.5, and HSP90.7 localized in different cellular compartments were involved in different functional mechanisms during oxidative stress.
- Subjects
PLANT genetics; DISEASE resistance of plants; MOLECULAR biology; ARABIDOPSIS thaliana; OXIDATIVE stress; OXIDATION-reduction reaction; HEAT shock proteins
- Publication
Plant Molecular Biology Reporter, 2009, Vol 27, Issue 3, p342
- ISSN
0735-9640
- Publication type
Article
- DOI
10.1007/s11105-009-0091-6