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- Title
Screening and molecular identification of biosurfactant/bioemulsifier producing bacteria from crude oil contaminated soils samples.
- Authors
Uyar, Ebru; Avcı, Tuba
- Abstract
A total of seventy-one bacteria were isolated from crude oil-contaminated soil samples. The isolates were screened for biosurfactant production by applying various methods, including determination of hemolytic, lipolytic, and esterolytic activities, oil displacement, parafilm M, drop-collapse, CTAB-methylene blue agar tests, determination of emulsification index (E24) and reduction in surface tension. Thirteen isolates were selected and further subjected to molecular identification. 16S rRNA gene sequencing analysis indicated that the isolates belong to Pseudomonas (Pseudomonas sp., P. balearica, and P. aeruginosa), Acinetobacter (A. radioresistens, A. baumanni), Bacillus (Bacillus sp.) and Priestia (Priestia sp.) genera. The largest spreading zone diameter (6.5 cm) and the highest enzymatic activity index (EAI) for lipolytic (5.4) and esterolytic (2.7) activities were demonstrated by P. aeruginosa strain TBA-B58. Surface tension analysis by the Du Noüy tensiometer confirmed that the glycolipid-type biosurfactant obtained from P. aeruginosa strain TBA-B58 achieved the highest reduction in surface tension with a value of 31.5 ± 0.5 mN m−1. Notable E24 values, superior to the synthetic surfactant sodium dodecyl sulfate, were recorded by the cell-free supernatants obtained from A. baumanni TBA-B45 (66%) and TBA-B56 (65%) against toluene and benzene, respectively. Based on the results of the present study, two biosurfactant/bioemulsifier-producing strains belonging to Pseudomonas and Acinetobacter genera demonstrated significant surface and interfacial activities, which might find applications in the bioremediation of crude oil hydrocarbons.
- Subjects
BIOSURFACTANTS; PETROLEUM; SOIL sampling; SODIUM dodecyl sulfate; SURFACE tension; MOUNTAIN soils; BACILLUS (Bacteria)
- Publication
Biologia, 2023, Vol 78, Issue 8, p2179
- ISSN
0006-3088
- Publication type
Article
- DOI
10.1007/s11756-023-01330-9