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- Title
Investigation of the protective and therapeutic effects of thiamine in thioacetamide-induced liver injury.
- Authors
Agirca Tasan, Serife; Ozmen, Ozlem
- Abstract
The purpose of this study was to evaluate the therapeutic and prophylactic effects of thiamine in mice with thioacetamide (TAA)-induced liver damage. In this study, 50 male CD-1 mice were used, and the study lasted 2 months. Group 1 was designated as the control group and given drinking water for 2 months. In addition, group 2 received 50 mg/100 mL of thiamine in water daily for 2 months. Group 3 received the same dosage of thiamine in water for 1 month and then 200 mg/kg of TAA intraperitoneally twice a week for 1 month. Group 4 received TAA for 1 month followed by thiamine in water ad libitum, and Group 5 was administered TAA for 1 month. At the end of the study, the mice were anesthetized and decapitated. Liver samples were collected for histopathological examination and blood samples for the evaluation of biochemical parameters. During necropsy, the liver samples from Group 5 were pale and swollen. Furthermore, histopathological changes were evaluated, and caspase-3 expression was examined immunohistochemically in the liver samples. It was found that all the parameters examined in the liver function test using serum samples increased significantly in Group 5. Therefore, TAA caused severe liver degeneration and necrosis. When thiamine was given after TAA, it improved the biochemical, pathological, and immunohistochemical findings. The improvement was more prominent in Group 4 than in Group 3. According to the results of this study, thiamine has a protective effect against cell damage on many cells, as well as a minor but significant therapeutic effect on liver cells against TAA toxicity.
- Subjects
VITAMIN B1; TREATMENT effectiveness; LIVER injuries; LIVER cells; THIAMIN pyrophosphate; LIVER function tests
- Publication
Biologia, 2022, Vol 77, Issue 7, p1953
- ISSN
0006-3088
- Publication type
Article
- DOI
10.1007/s11756-022-01062-2